THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Receive recombinant DNA for cloning purpose
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Join DNA fragments permanently between gene of interest and plasmid vector forming recombinant
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Produce the desired protein
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Recognize and cut the DNA at specific base sequences called restriction sites
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Detailed explanation-1: -Restriction enzymes and DNA ligases represent the cutting and joining functions in DNA manipulation. All other enzymes involved in genetic engineering fall under the broad category of enzymes known as DNA modifying enzymes. These enzymes are involved in the degradation, synthesis and alteration of the nucleic acids.
Detailed explanation-2: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.
Detailed explanation-3: -Ligase is an enzyme that is able to join two different fragments of DNA together. The sticky ends of the DNA fragments are complementary to each other, allowing ligase to bind them together, sealing the gene into the plasmid.
Detailed explanation-4: -The enzyme DNA ligase joins the fragments with matching ends together to form a single, unbroken molecule of DNA. This produces a recombinant plasmid that contains the target gene.