BOTANY
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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3
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6
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5
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4
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Detailed explanation-1: -If a linear DNA molecule is digested with a restriction enzyme at four recognition sites on the DNA, 5 fragments would be produced.
Detailed explanation-2: -These restriction enzymes cleave the plasmid at specific sites. On cleaving a circular DNA 3 fragments of DNA will be obtained as there are 3 recognition sites for restriction enzyme.
Detailed explanation-3: -When a closed circular DNA molecule is digested with a restriction enzyme having six recognition sites, it will produce 6 DNA fragments.
Detailed explanation-4: -The image processing system, LabEye Profile, calculates the size of DNA restriction fragments according to the formula MD = f(x); x = 1/log bp. The migration distances (MD) of known fragment sizes are interpolated by the use of cubic splines. Cubic splines are also used to correct for gel distortions.