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Detailed explanation-1: -This requirement makes it possible to delineate a specific region of template sequence that the researcher wants to amplify. At the end of the PCR reaction, the specific sequence will be accumulated in billions of copies (amplicons).

Detailed explanation-2: -Polymerase chain reaction is commonly known as PCR. It is used to produce multiple copies of a gene or DNA of interest. The steps of polymerase chain reaction are denaturation, primer annealing and extension of primers.

Detailed explanation-3: -PCR (polymerase chain reaction) tests are a fast, highly accurate way to diagnose certain infectious diseases and genetic changes. The tests work by finding the DNA or RNA of a pathogen (disease-causing organism) or abnormal cells in a sample.

Detailed explanation-4: -Sometimes called “molecular photocopying, ” the polymerase chain reaction (PCR) is a fast and inexpensive technique used to “amplify”-copy-small segments of DNA.

Detailed explanation-5: -PCR stands for polymerase chain reaction. It is a process which is used to create copies of DNA fragment. In this, PCR reaction mixture contains Taq polymerase, primers, template DNA and nucleotides There are three steps of PCR namely, denaturation step, annealing step and extension step.