BOTANY

Detailed explanation-1: -The restriction endonuclease enzyme EcoRI recognizes the ssDNA sequence 5’-GAATTC’-3, and introduces a single-strand cut between the G & A nucleotides. This recognition site is a palindrome: the opposite strand also reads 5’-GAATTC’-3 and will be cut in the same manner.

Detailed explanation-2: -EcoRI has a palindrome recognition site that cuts DNA following G, creating sticky ends with AATT. It is a six-nucleotide sequence with GAATTC in the 5’ to 3’ direction and CTTAAG in the 3’ to 5’ direction on the complementary strand.

Detailed explanation-3: -EcoRI recognizes the sequence GAATTC, and cuts both DNA strands between the G and the A nucleotides. Protruding from the cut ends will be single-stranded DNA “tails” having the sequences AATT.

Detailed explanation-4: -This is a palindrome. Therefore, the sequence that EcoRI recognizes in the five prime to three prime direction is GAATTC.

Detailed explanation-5: -The restriction enzyme EcoRI was first isolated by Herbert Boyer in 1969 from E. coli. It cleaves the DNA between G and A in the base sequence GAATTC.