BOTANY

Detailed explanation-1: -Restriction enzymes cleave DNA at specific nucleotide sequences. Restriction endonucleases cleave double-stranded DNA.

Detailed explanation-2: -Restriction enzymes are DNA-cutting enzymes. Each enzyme recognizes one or a few target sequences and cuts DNA at or near those sequences. Many restriction enzymes make staggered cuts, producing ends with single-stranded DNA overhangs.

Detailed explanation-3: -In the laboratory, restriction enzymes (or restriction endonucleases) are used to cut DNA into smaller fragments. The cuts are always made at specific nucleotide sequences. Different restriction enzymes recognise and cut different DNA sequences.

Detailed explanation-4: -Restriction enzymes are proteins.

Detailed explanation-5: -The restriction enzymes generate two different types of cuts. Blunt ends are produced when they cut the DNA at the centre of the recognition sequence, and sticky ends produce an overhang.