BOTANY

Detailed explanation-1: -The preparation includes cutting the selected source of explant to a suitable size that should fulfill the goal of culturing. The explants are pretreated and sterilized with certain chemicals before they are inoculated in the growth medium.

Detailed explanation-2: -Micropropagation Stage III-Root formation In the rooting stage, microcuttings are induced to form roots-usually by application of auxin. In general, species root easier in tissue culture than they do from conventional cuttings. Microcuttings can either be rooted in vitro or ex vitro.

Detailed explanation-3: -It is in this stage, the major activity of micropropagation occurs in a defined culture medium. Stage II mainly involves the multiplication of shoots or rapid embryo formation from the explant. A growth chamber set at 20–24 °C is used, with a 2000-to 4000-lux light intensity, and a lighting period of 16 hours or so.

Detailed explanation-4: -Initiation Phase: The tissue is initiated in the culture at this time. The tissue of interest is acquired, introduced, and sterilized to avoid contamination. Multiplication Phase: The sterile explant is placed in a medium containing growth regulators and required nutrients.