In the wet curing preparation method, sometimes a drop of Toluidine Blue solution is placed on the incision of the plant tissue which functions to color the part of the plant.

PLANT MORPHOLOGY

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Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]

A	lignin
B	cell wall
C	cytoplasm
D	organelles

Explanation:

Detailed explanation-1: -APPLICATION OF TOLUIDINE BLUE Mast cell granules stain purple in color due to the presence of heparin and histamine.

Detailed explanation-2: -It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images. It is especially useful today for staining chromosomes in plant or animal tissues, as a replacement for Aceto-orcein stain.

Detailed explanation-3: -TBO provides a pinkish purple color with carboxylated polysaccharides such as pectic acid; green, greenish blue or bright blue with polyphenolic substances such as lignin and tannins; and purplish or greenish blue with nucleic acids. Staining is more intense at low pHs (2.5–3.5) (Preece 1972: 266).

Detailed explanation-4: -Complete answer: Methylene blue: Methylene blue is a common laboratory stain and is used for many different types of cells.

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PLANT MORPHOLOGY