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Detailed explanation-1: -Agitation of the medium also exerts a mild pressure on tissue and breaks it into smaller cell pieces and single cells. This agitation helps to maintain the uniform distribution and movement of cells in the medium. Thus, it is an important procedure for cell suspension culture.

Detailed explanation-2: -Accordingly, cell suspension cultures for commercial use have been studied recently. Examples of uses for these cultures include secondary metabolite production for drugs, flavorings and fragrances, and micropropagation, including the production of somatic embryos.

Detailed explanation-3: -Suspension cultures can be maintained in sterile culture flasks (e.g., shaker flasks without baffles) that are not tissue-culture treated; however, spinner flasks (i.e., stirrer bottles) specifically designed for suspension cell culture allow for superior gas exchange and permit higher volumes of cells to be cultured.