COMPUTER FUNDAMENTALS

COMPUTER APPLICATIONS AND USES

SCIENTIFIC AND ENGINEERING APPLICATIONS

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?
A
DNA is negatively charged so attracted to the positive end of the unit
B
DNA is positively charged to attracted to the negative end of the unit
C
the agarose gel in negatively charged
D
the agarose gel is positively charged
Explanation: 

Detailed explanation-1: -DNA fragments are negatively charged, so they move towards the positive electrode. Because all DNA fragments have the same amount of charge per mass, small fragments move through the gel faster than large ones.

Detailed explanation-2: -This is due to negative charges on the phosphate groups of its nucleotides. As a result, DNA can be pulled toward a positive charge. This is how gel electrophoresis pulls DNA through an agarose gel. Due to its negatively charged backbone, DNA is strongly attracted to a positive electrode.

Detailed explanation-3: -DNA consist of a phosphate backbone which is a negatively charged, hence when the DNA is placed in gei-electrophoresis it always moves towards anode, as the anode is positively charged.

There is 1 question to complete.