MCQ IN COMPUTER SCIENCE & ENGINEERING

Detailed explanation-1: -Colorimetry is a scientific technique that is used to determine the concentration of colored compounds in solutions by the application of the Beer–Lambert law, which states that the concentration of a solute is proportional to the absorbance.

Detailed explanation-2: -Colorimetric assays use a colorimeter (spectrophotometer) to determine concentration of a chemical compound in a solution by measuring spectral absorbance of the compound at a particular wavelength.

Detailed explanation-3: -Colorimetric analysis is the technique normally used to determine the concentration of analyte through comparing the color changes of the solution.

Detailed explanation-4: -The filters are used to isolate a part of the visible light spectrum that is absorbed maximally by the sample. Different colourimeters use different sets of filters but typical wavelengths passed are red filter: 630-750nm, green filter: 510-570nm and blue filter: 360-480nm.

Detailed explanation-5: -A colorimeter is a light-sensing device that is used to measure the absorbance and transmittance of light as it moves through a sample of liquid. The main components of a colorimeter include: Illuminant: A specific, fixed light source that passes through the object.