DENDROLOGY

TREE PROPAGATION AND PLANTING

PLANTING TECHNIQUES

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Agitation is normally applied in suspension culture to I. avoid contamination. II. provide gas exchange. III. breakdown of cell aggregates into small clumps of cells. IV. maintain a uniform distribution of cells and clump cells in the medium.
A
I, II and III
B
I, II and IV
C
II, III and IV
D
All of the above.
Explanation: 

Detailed explanation-1: -Examples of uses for these cultures include secondary metabolite production for drugs, flavorings and fragrances, and micropropagation, including the production of somatic embryos. In the latter case, highly efficient propagation can be achieved using cell suspension cultures.

Detailed explanation-2: -Characteristics and advantages of Suspension cell culture Large-scale suspension cultures are constantly agitated to allow for adequate gas and nutrient exchange. This is primarily achieved by the use of a magnetic stirrer or rotating spinner flasks.

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