EVERYDAY SCIENCE

Detailed explanation-1: -Plant tissue culture media should generally contain some or all of the following components: macronutrients, micronutrients, vitamins, amino acids or nitrogen supplements, source(s) of carbon, undefined organic supplements, growth regulators and solidifying agents.

Detailed explanation-2: -Plant tissue and cell culture media are generally made up of some or all of the following components: macronutrients, micronutrients, vitamins, amino acids or other nitrogen supplements, sugar(s), other undefined organic supplements, solidifying agents or support systems, and growth regulators.

Detailed explanation-3: -Essential components of cell culture media include carbohydrates, amino acids, vitamins, minerals, and a pH buffer system. Your choice of media can affect the accuracy, reproducibility, and relevance of your experimental results.

Detailed explanation-4: -Reconstitute medium by adding required quantity of powder in two-third of total volume with constant, gentle stirring till the medium gets completely dissolved. Add heat stable supplements prior to autoclaving. Make up the final volume with distilled water. Adjust the pH of the medium to 5.75 ± 0.5 using 1N NaOH/ HCl.