SCIENCE
BOTANY
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Question
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Induction of direct regeneration of shoots from organ cultures.
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Induction of direct regeneration of roots from organ cultures.
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Induction of direct regeneration of stomata from organ cultures.
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Induction of direct regeneration of xilem from organ cultures.
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Detailed explanation-1: -During tissue culture, adventitious roots or shoots can be induced by transferring the callus to medium containing different ratios of auxin and cytokinin. In some cases in nature, de novo organogenesis from detached organs leads directly to the formation of a new plant at a wound site, without production of callus.
Detailed explanation-2: -In direct regeneration, in vitro organs are directly induced from explant tissues; in indirect regeneration, a de novo organ is typically formed from an intermediate tissue, the callus.
Detailed explanation-3: -Introduction. Micropropagation is a method of plant propagation using extremely small pieces of plant tissue taken from a carefully chosen and prepared mother plant, and growing these under laboratory conditions to produce new plants. It is widely used in commercial horticulture.
Detailed explanation-4: -The stages of plant tissue culture are: selection/preparation (stage 0), initiation/establishment (stage 1), multiplication (stage 2), rooting (stage 3), and acclimatization/hardening (stage 4).