SCIENCE
BOTANY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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To detect PCR products as they are produced.
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To quantify PCR products
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To detect a viral load
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To amplify RNA viruses
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Detailed explanation-1: -This means PCR is used for pathogens, such as viruses and bacteria, that already contain DNA for amplification, while RT–PCR is used for those containing RNA that needs to be transcribed to DNA for amplification.
Detailed explanation-2: -The use of the polymerase chain reaction (PCR) in molecular diagnostics has increased to the point where it is now accepted as the gold standard for detecting nucleic acids from a number of origins and it has become an essential tool in the research laboratory.
Detailed explanation-3: -The Polymerase Chain Reaction Reverse transcription (RT)-PCR is used to amplify RNA targets. The RNA template is converted into complementary (c)DNA by the enzyme reverse transcriptase.
Detailed explanation-4: -Compared to the two other commonly used techniques for quantifying mRNA levels, Northern blot analysis and RNase protection assay, RT-PCR can be used to quantify mRNA levels from much smaller samples. In fact, this technique is sensitive enough to enable quantitation of RNA from a single cell.
Detailed explanation-5: -It uses an enzyme called reverse transcriptase to change a specific piece of RNA into a matching piece of DNA. This piece of DNA is then amplified (made in large numbers) by another enzyme called DNA polymerase. The amplified DNA copies help tell whether a specific mRNA molecule is being made by a gene.