SCIENCE
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Question
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Branched DNA
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Nested PCR
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Real time PCR
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Reverse Transcriptase PCR
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Detailed explanation-1: -Real-time multiplex PCR uses a set of species-specific primers and probe that is labeled with different fluorescent dyes for each target species so that approximately two to five species (depending on the experimental conditions) can be detected simultaneously in a single real-time PCR reaction.
Detailed explanation-2: -Real-time polymerase chain reaction (real-time PCR) is commonly used to measure gene expression. It is more sensitive than microarrays in detecting small changes in expression but requires more input RNA and is less adaptable to high-throughput studies (1).
Detailed explanation-3: -Multiplex-PCR may be used, for example, for identifying various serotypes of Shiga toxin-producing E. coli (Svoboda et al., 2013). Multiplex PCR was also used for serotyping L. monocytogenes, by detecting four genes corresponding to serotypes 1/2a, 1/2b, 1/2c, and 4b.
Detailed explanation-4: -First, add the master mix or dNTPs cocktail to the reaction tube. Add reaction or multiplex buffer to that. Add sets or primers simultaneously to the reaction. Add Probe or dye, if the assay is quantitative. Add the template DNA to the reaction. More items •01-Mar-2019