SCIENCE
BOTANY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Annealing
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Initiation
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Denaturation
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Extension
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Detailed explanation-1: -In this step, heat is applied to the template to separate double-stranded DNA into two single strands. Following the denaturation step is the annealing step. The temperature is decreased so that the primers can anneal to the complementary sequences on the DNA templates.
Detailed explanation-2: -During the second step of PCR, called the annealing step, the primers attach, or anneal, to the DNA template. PCR requires two different primers, one that can attach to each strand of the DNA molecule.
Detailed explanation-3: -Annealing: The temperature of the reaction is lowered to 54 to 60° C for about 20 to 40 seconds. In this step, the primers bind to the complementary sequences on the DNA template. Elongation: At this step of the reaction, the temperature is increased to 72 to 80° C.