EVERYDAY SCIENCE

SCIENCE

CELL BIOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
A student observed palisade cells using a microscope. The microscope had four objective lenses, each with a different magnification. Which objective lens should the student use first?
A
×4 magnification
B
×10 magnification
C
×40 magnification
D
×100 magnification
Explanation: 

Detailed explanation-1: -Explanation: The student should use the x4 objective lense in order to be able to observe the palisade cell of a leaf. The palisade cells are located below the cuticle and the epidermis of the leaf and the x4 objective will provide the largest field of view for adequate study by the student.

Detailed explanation-2: -When using a light microscope it’s important to start with the low power objective lens as the field of view will be wider, increasing the number of cells you are able to see. This makes it easier to find what you’re looking for.

Detailed explanation-3: -Below the epidermis (top layer of cells), are two layers of column-like cells known as palisade cells. These contain large numbers of chloroplasts and are the principal sites of photosynthesis. Magnification: x240 at 35mm size.

Detailed explanation-4: -Magnification: Your microscope has 4 objective lenses: Scanning (4x), Low (10x), High (40x), and Oil Immersion (100x).

Detailed explanation-5: -Why do you need to start with 4x in magnification on a microscope? The 4x objective lens has the lowest power and, therefore the highest field of view. As a result, it is easier to locate the specimen on the slide than if you start with a higher power objective.

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