EVERYDAY SCIENCE

Detailed explanation-1: -So, the correct answer is ‘Differential and density gradient centrifugation’

Detailed explanation-2: -In this method, subcellular organelles are separated by centrifugation through a gradient of a dense substance, such as sucrose. In velocity centrifugation, the material to be separated is layered on top of a sucrose gradient, and then centrifuged.

Detailed explanation-3: -The ultracentrifuge is also used to separate cellular components on the basis of their buoyant density, independently of their size and shape.

Detailed explanation-4: -In biochemistry and cell biology, differential centrifugation (also known as differential velocity centrifugation) is a common procedure used to separate organelles and other sub-cellular particles based on their sedimentation rate.

Detailed explanation-5: -Differential centrifugation is a common procedure in microbiology and cytology used to separate certain organelles from whole cells for further analysis of specific parts of cells. In the process, a tissue sample is first homogenised to break the cell membranes and mix up the cell contents.