EVERYDAY SCIENCE

Detailed explanation-1: -Louis Pasteur’s 1859 experiment showed that a boiled nutrient broth did not give rise spontaneously to new life, but that if direct access to air was permitted, the broth decomposed, implying that small organisms (in modern terms, microbial spores) had fallen in and started to grow in the broth.

Detailed explanation-2: -Disproving Spontaneous Generation In 1858, Pasteur filtered air through a gun-cotton filter and, upon microscopic examination of the cotton, found it full of microorganisms, suggesting that the exposure of a broth to air was not introducing a “life force” to the broth but rather airborne microorganisms.

Detailed explanation-3: -These experiments proved that there was no spontaneous generation, since the boiled broth, if never reexposed to air, remained sterile.