SCIENCE
GENETIC AND MENDELS LAW OF HEREDITY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Plasmid
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Vector
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Cloning vehicle
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Both b and c
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Detailed explanation-1: -In practice the procedure is carried out by inserting a DNA fragment into a small DNA molecule and then allowing this molecule to replicate inside a simple living cell such as a bacterium. The small replicating molecule is called a DNA vector (carrier).
Detailed explanation-2: -Recombinant DNA technology involves using enzymes and various laboratory techniques to manipulate and isolate DNA segments of interest. This method can be used to combine (or splice) DNA from different species or to create genes with new functions. The resulting copies are often referred to as recombinant DNA.
Detailed explanation-3: -In a typical DNA cloning procedure, the gene or other DNA fragment of interest (perhaps a gene for a medically important human protein) is first inserted into a circular piece of DNA called a plasmid.
Detailed explanation-4: -Recombinant DNA (rDNA) technology refers to the process of joining DNA molecules from two different sources and inserting them into a host organism, to generate products for human use.
Detailed explanation-5: -In molecular cloning with bacteria, a desired DNA fragment is inserted into a bacterial plasmid using restriction enzymes and the plasmid is taken up by a bacterium, which will then express the foreign DNA.