EVERYDAY SCIENCE

Detailed explanation-1: -In practical terms, bacteria and mitochondria, which are about 500 nm (0.5 m) wide, are generally the smallest objects whose shape can be clearly discerned in the light microscope; details smaller than this are obscured by effects resulting from the wave nature of light.

Detailed explanation-2: -Bacteria are too small to see without the aid of a microscope. While some eucaryotes, such as protozoa, algae and yeast, can be seen at magnifications of 200X-400X, most bacteria can only be seen with 1000X magnification. This requires a 100X oil immersion objective and 10X eyepieces..

Detailed explanation-3: -If you’re interested in getting a good look at bacteria, you’re going to need a compound microscope with a high level of magnification. Most bacteria are small and hard to see, with an average size somewhere between 0.2 and 2.0 m (micrometers).

Detailed explanation-4: -Compound microscopes are especially useful for seeing the internal structure of specimens and are often used to see structures (e.g., organelles) within cells. A dissecting microscope shines light on the surface of a specimen and produces a binocular (3D) image.

Detailed explanation-5: -With higher levels of magnification than stereo microscopes, a compound microscope uses a compound lens to view specimens which cannot be seen at lower magnification, such as cell structures, blood, or water organisms.