EVERYDAY SCIENCE

Detailed explanation-1: -Gram-negative bacteria are surrounded by a thin peptidoglycan cell wall, which itself is surrounded by an outer membrane containing lipopolysaccharide. Gram-positive bacteria lack an outer membrane but are surrounded by layers of peptidoglycan many times thicker than is found in the Gram-negatives.

Detailed explanation-2: -If the bacteria are gram-positive, the thick, peptidoglycan layer in their cell walls will retain the dye and they will stain violet. If the bacteria are gram-negative, the dye will leak out of the thin peptidoglycan layer, and the bacteria will stain red.

Detailed explanation-3: -A Gram-positive bacterium has a cell wall in which chains of amino acids are linked together by groups of four sugars, while Gram-negative bacteria have chains of sugars linked together by groups of amino acids.

Detailed explanation-4: -Most Gram-positive bacteria have a relatively thick (about 20 to 80 nm), continuous cell wall (often called the sacculus), which is composed largely of peptidoglycan (also known as mucopeptide or murein).

Detailed explanation-5: -Gram-positive bacteria cell wall contains a thick peptidoglycan cell wall along with teichoic acid whereas Gram-negative bacteria cell contains a thin peptidoglycan cell wall with no teichoic acid, so it makes the color of the cell of both different (Panawala, 2017) .