LIFE SCIENCE

Detailed explanation-1: -Process: Dilution equation (C1V1 = C2V2) = 0.005 mL (5 µL) of the concentrated solution is needed. Add 5mM NaCl solution and make 500 µL of 50µM NaCl solution, then take 0.005 mL (5 µL) of the concentrated stock solution and 0.495 mL (495 µL) of solvent.

Detailed explanation-2: -A buffer solution can protect the integrity of the proteins while separating them from other integrated cell components. To accomplish this goal, researchers need to choose a buffer solution that’s compatible with the protein in question and recreates an ionic environment similar to the ionic environment of the cell.