OBJECTIVE LIFE SCIENCE
APPLIED BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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two somatic cells
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two gamete cells
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an egg cell and a somatic cell
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a sperm cell and a somatic cell
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Detailed explanation-1: -Cloning using somatic cell nuclear transfer (SCNT) [ 1]. This procedure starts with the removal of the chromosomes from an egg to create an enucleated egg. The chromosomes are replaced with a nucleus taken from a somatic (body) cell of the individual or embryo to be cloned.
Detailed explanation-2: -Once inside the egg, the somatic nucleus is reprogrammed by egg cytoplasmic factors to become a zygote (fertilized egg) nucleus. The egg is allowed to develop to the blastocyst stage, at which point a culture of embryonic stem cells (ESCs) can be created from the inner cell mass of the blastocyst.
Detailed explanation-3: -Today most cloning is performed with plasmid vectors. The plasmid vectors most widely used for gene cloning are small circular molecules of double-stranded DNA derived from larger plasmids that occur naturally in bacterial cells.