OBJECTIVE LIFE SCIENCE
BIOCHEMISTRY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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an increase in solubility called ‘salting in’.
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a decrease in solubility called ‘salting out’.
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protein precipitation from solution.
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all of the above
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none of the above
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Detailed explanation-1: -For example, the addition of salts weakens electrostatic interactions by screening charges. This means that at pH values where the protein is strongly charged, the addition of salt reduces electrostatic repulsion, decreasing the solubility (salting out).
Detailed explanation-2: -Using a high-salt concentration loading buffer is crucial in ensuring the high purity of the resulting protein product. High salt decreases non-specific binding between the protein of interest and impurities by interrupting the electrostatic interactions between them.
Detailed explanation-3: -When salt is added to the solution, the surface tension of the water increases, resulting in increased hydrophobic interaction between protein and water.
Detailed explanation-4: -During the salting in process, salt molecules increase the solubility of proteins by reducing the electrostatic interactions between protein molecules.