LIFE SCIENCE

Detailed explanation-1: -Denaturation of the enzymes generally refers to conformational changes in protein structure & active site of the enzyme and also results in the inactivity of enzymes. Factors that can denature an enzyme are: Enzymes usually require an optimum temperature and pH for their action.

Detailed explanation-2: -Temperature: Raising temperature generally speeds up a reaction, and lowering temperature slows down a reaction. However, extreme high temperatures can cause an enzyme to lose its shape (denature) and stop working. pH: Each enzyme has an optimum pH range. Changing the pH outside of this range will slow enzyme activity.

Detailed explanation-3: -Extremes of pH also denature enzymes. The changes are usually, though not always, permanent. Enzymes work inside and outside cells, for instance in the digestive system where cell pH is kept at 7.0pH to 7.4pH. Cellular enzymes will work best within this pH range.