LIFE SCIENCE

Detailed explanation-1: -Dinitrogenase reductase is a dimeric (2) Fe-protein of about 70 kDa with a 4Fe–4S cluster, which binds ATP and transfers electrons to dinitrogenase. The latter enzyme is a tetrameric (22) FeMo protein of about 220 kDa. It contains two Mo–Fe–S clusters and a variable number of Fe–S clusters and binds N2.

Detailed explanation-2: -The enzyme is composed of two-component metalloproteins called the iron (Fe) protein (also called dinitrogenase reductase or component II) and the molybdenum-iron (MoFe) protein (also called dinitrogenase or component I) (Figure 1).

Detailed explanation-3: -Dinitrogenase reductase transfers electrons from an electron donor to dinitrogenase, which then can catalyze the reduction of nitrogen to ammonium. Because nitrogen fixation is an energy-demanding process, it is tightly regulated in response to a number of environmental factors.

Detailed explanation-4: -Nitrogenase consists of two component metalloproteins, the iron (Fe) protein and the molybdenum-iron (MoFe) protein [or the homologous alternative vanadium (VFe) and iron-only (FeFe) nitrogenases].

Detailed explanation-5: -Nitrogenase is a complex, bacterial enzyme that catalyzes the ATP-dependent reduction of dinitrogen (N2) to ammonia (NH3). In its most prevalent form, it consists of two proteins, the catalytic molybdenum-iron protein (MoFeP) and its specific reductase, the iron protein (FeP).