LIFE SCIENCE

Detailed explanation-1: -The amino acid to be replaced is glutamic acid, Glu, which is one of the two amino acids that are negatively charged. So it is expected for a conservative substitution to happen when the other negatively charged amino acid replaces glutamic acid.

Detailed explanation-2: -Conservative substitution: The replacement of an amino acid residue in one protein by one with similar chemical properties in another protein. For example, the replacement of serine by threonine, or leucine by isoleucine, are both considered conservative substitutions.

Detailed explanation-3: -Generally amino acid residues located on the surface of a protein serve as active sites and/or interact with other molecules and ligands [9].

Detailed explanation-4: -Alanine is a conservative substitution for glycine.

Detailed explanation-5: -The amino acids found on the interior of the globular protein are isoleucine and phenylalanine. The amino acids found on the surface are aspartic acid, lysine, arginine, and glutamic acid.