OBJECTIVE LIFE SCIENCE
BIOCHEMISTRY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Research scientists are trying to clone a gene. In order to accomplish this task they join two pieces of DNA. Which class of the enzymes below might accomplish this task?
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oxidoreductase
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ligase
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hydrolase
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isomerase
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lyase
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Explanation:
Detailed explanation-1: -DNA ligase enzymes join two different fragments of DNA by forming phosphodiester bonds. Research scientists used this method for the cloning of genes.
Detailed explanation-2: -To join sticky ends DNA ligase enzyme is used which acts by catalyzing the formation of a phosphodiester bond. DNA ligases are called Molecular glue since these enzymes are used during replication for joining phosphodiester backbone, used in RDT for joining sticky ends, and also used in damage and repair cells.
Detailed explanation-3: -isolation of the DNA of interest (or target DNA), ligation, transfection (or transformation), and. a screening/selection procedure. 05-Dec-2014
There is 1 question to complete.