LIFE SCIENCE

OBJECTIVE LIFE SCIENCE

BIOCHEMISTRY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
What is the isoelectric point?
A
The pH in which the amino acid is a zwitterion ion a
B
The resultant electrostatic charge is equal to 0
C
The pH in which the amino acid is a zwitterion ion and the resultant electrostatic charge is equal to 0
D
The pH in which the amino acid is a zwitterion ion and the resultant electrostatic charge is equal to 5
Explanation: 

Detailed explanation-1: -At a certain pH of the medium called an isoelectric point of an -amino acid, the Zwitterion behaves as neutral and does not migrate to any electrode on passing current. The isoelectric points are in the pH range of 5. 5 to 6.

Detailed explanation-2: -Another main property of a Zwitterion is that it has an isoelectric point (represented as pI, pH(I), IEP). This point is the pH value at which the charge in molecules is neutral. Usually, the net charge on a molecule is greatly affected by the pH of its surrounding environment.

Detailed explanation-3: -The particular pH at which a given amino acid exists in solution as a zwitterion is called the isoelectric point (pI). At its pI, the positive and negative charges on the amino acid balance, and the molecule as a whole is electrically neutral.

Detailed explanation-4: -The zwitterion is an ion or molecule that contains an equal number of positively and negatively charged functional groups. At isoelectric point, the concentration of zwitterions is maximum. Thus, the statement ‘for neutral amino acid, the concentration of zwitterion is maximum at its isoelectric point’ is correct.

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