LIFE SCIENCE

Detailed explanation-1: -Phosphofructokinase (PFK) utilizes ATP to phosphorylate fructose-6-phosphate to fructose-1, 6-bisphosphate . As a regulatory enzyme of glycolysis, PFK is negatively inhibited by ATP and citrate and positively regulated by ADP.

Detailed explanation-2: -Important features of glycolysis are discussed as follows: An enzyme called hexokinase uses the energy of ATP to add a phosphate group to glucose to form glucose-6-phosphate. Similarly, an enzyme, called phosphofructokinase, uses ATP to add a phosphate group to fructose-6-phosphate to form fructose-1, 6-diphosphate.

Detailed explanation-3: -The third step is the phosphorylation of fructose-6-phosphate, catalyzed by the enzyme phosphofructokinase.

Detailed explanation-4: -Phosphofructokinase-1 (EC:2.7. 1.11, PFK-1) catalyzes the phosphorylation of fructose 6-phosphate to fructose 1, 6-bisphosphate using adenosine triphosphate and is a key regulatory enzyme of glycolysis. Mammalian PFK-1 isozymes are composed of three kinds of subunits (PFK-M, -L, and-P), with different properties.

Detailed explanation-5: -Description. Phosphofructokinase (PFK) catalyses the phosphorylation of fructose-6-phosphate to fructose-1, 6-biphosphate, which then enters the Embden-Meyerhof pathway. PFK is a key regulatory enzyme in glycolysis.