LIFE SCIENCE

Detailed explanation-1: -The secondary structure arises from the hydrogen bonds formed between atoms of the polypeptide backbone. The hydrogen bonds form between the partially negative oxygen atom and the partially positive nitrogen atom.

Detailed explanation-2: -Our results indicate that in a domain lacking secondary structure, disulfides form before conformational folding through a process prone to nonnative disulfide formation, whereas in proteins with defined secondary structure, native disulfide formation occurs after partial folding.

Detailed explanation-3: -Disulfide bonds are a central structural element which stabilizes the mature proteins’ 3D structure and/or exhibit physiologically relevant redox activity (Bosnjak et al., 2014). They are mostly found in secretory proteins and extracellular domains of membrane proteins.

Detailed explanation-4: -During the denaturation process of protein, the secondary and tertiary structure of protein will be changed but the peptide bond of the primary structure remains constant.