OBJECTIVE LIFE SCIENCE
BIOCHEMISTRY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
solubility
|
|
stereochemistry
|
|
size
|
|
charge
|
|
polarity
|
Detailed explanation-1: -Proteins are separated on the basis of differences in their physicochemical properties, such as size, charge, adsorption characteristics, solubility and heat-stability.
Detailed explanation-2: -Two chromatographic methods are frequently used for protein or (more often) peptide separation. High-performance liquid chromatography (HPLC) can be used to separate and to purify proteins/peptides based on size, charge or overall hydrophobicity.
Detailed explanation-3: -Adsorption chromatography is not widely used in protein purification. Hydroxyapatite is an example of a stationary phase that is sometimes used to analyze either proteins or nucleic acids. Ion exchange chromatography (IEC) is a specific type of adsorption chromatography based upon charge-charge interactions.