LIFE SCIENCE

Detailed explanation-1: -Thus, a sample of proteins passing through a gel filtration column will separate based on molecular size: The big ones will elute first and the smallest ones will elute last (and “middle” sized proteins will elute in the middle).

Detailed explanation-2: -Gel filtration (GF) chromatography separates proteins solely on the basis of molecular size.

Detailed explanation-3: -Therefore, immunoglobulin G should elute second in size-exclusion chromatography.

Detailed explanation-4: -Gel filtration chromatography can be used to separate compounds such as small molecules, proteins, protein complexes, polysaccharides, and nucleic acids when in aqueous solution. When an organic solvent is used as the mobile phase, the process is instead referred to as gel permeation chromatography.