LIFE SCIENCE

OBJECTIVE LIFE SCIENCE

BIOTECHNOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be
A
human gene may have intron which bacteria cannot process
B
amino acid codons for humans and bacteria are different
C
human protein is formed but degraded by bacteria
D
all of the above
Explanation: 

Detailed explanation-1: -So, the correct answer is ‘Human gene may have intron which bacteria cannot process’

Detailed explanation-2: -During transformation, a bacterial cell takes up the plasmid. Afterward, that bacterial cell will contain the genes in that plasmid and can express those genes. One example of a transgenic microorganism is the bacterial strain that produces human insulin. The insulin gene from humans was inserted into a plasmid.

Detailed explanation-3: -A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be human gene may have intron which bacteria cannot process.

Detailed explanation-4: -For transformation with recombinant DNA, the bacterial cells must first be made competent which means increasing the efficiency with which the DNA enters the bacterium.

There is 1 question to complete.