OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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ori
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selectable marker
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both of above
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non of these
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Detailed explanation-1: -A cloning vector generally refers to a small DNA fragment into which a foreign DNA of interest can be inserted. The vector DNA should be of a small size. It must be self-replicating inside the host cell. The vector DNA must have an ori(locus) and restriction site.
Detailed explanation-2: -A cloning vector should possess an origin of replication so that it can self-replicate inside the host cell. It should have a restriction site for the insertion of the target DNA. It should have a selectable marker with an antibiotic resistance gene that facilitates screening of the recombinant organism.
Detailed explanation-3: -The different types of vectors available for cloning are plasmids, bacteriophages, bacterial artificial chromosomes (BACs), yeast artificial chromosomes (YACs) and mammalian artificial chromosomes (MACs).