OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Assertion-E.coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracyclineReason-Recognition site for Bam HI is present in tetr region of pBRE322
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if both assertion and reason are true and reason is the correct explanation of assertion
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if both assertion and reason are true but reason is not the correct explanation of assertion
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if assertion is true but reason is false
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if both assertion and reason are false
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Explanation:
Detailed explanation-1: -coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline. Reason: Recognition site for Bam HI is present in tetR region of pBR322. (a) Both assertion and reason are true, and the reason is the correct explanation of the assertion.
Detailed explanation-2: -The plasmid pBR322 vector carries the genes for tetracycline (tetR) and ampicillin (ampR) resistance. Ampicillin (ampR) resistance genes are PstI and PvuI. Tetracycline (tetR) resistance genes are BamHI and SalI. These genes are useful to identify and select the transformants and non-transformants.
Detailed explanation-3: -So, the correct answer is ‘BamHl’.
There is 1 question to complete.