OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Construction recombinant DNA by joining with cloning vectors
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Cutting of DNA into fragments
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separation of DNA fragments according to their size
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Isolation of DNA molecules
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Detailed explanation-1: -Gel electrophoresis is a technique used to separate DNA fragments according to their size. DNA samples are loaded into wells (indentations) at one end of a gel, and an electric current is applied to pull them through the gel. DNA fragments are negatively charged, so they move towards the positive electrode.
Detailed explanation-2: -Agarose gel analysis is the most commonly used method for analyzing DNA fragments between 0.1 and 25 kb, while pulse-field gel electrophoresis enables analysis of DNA fragments up to 10, 000 kb.
Detailed explanation-3: -The gel is placed in an electrophoresis chamber, which is then connected to a power source. When the electric field is applied, the larger molecules move more slowly through the gel while the smaller molecules move faster. The different sized molecules form distinct bands on the gel.