OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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How do you create recombinant DNA?
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First, cut the gene and plasmid with the same restriction enzyme. Second, insert the gene into the plasmid.
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First, insert the gene into the plasmid. Second, cut the gene and plasmid with the same restriction enzyme.
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First, cut the gene and plasmid with different restriction enzymes. Second, insert the gene into the plasmid.
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First, insert the gene into the plasmid. Second, cut the gene and plasmid with different restriction enzymes.
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Explanation:
Detailed explanation-1: -A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.
Detailed explanation-2: -Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends with a single-stranded overhang. If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase. DNA ligase seals the gap between the molecules, forming a single piece of DNA.
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