OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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2
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3
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4
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5
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6
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Detailed explanation-1: -These restriction enzymes cleave the plasmid at specific sites. On cleaving a circular DNA 3 fragments of DNA will be obtained as there are 3 recognition sites for restriction enzyme. Thus, the correct answer is option C.
Detailed explanation-2: -If a linear DNA molecule is digested with a restriction enzyme at four recognition sites, 4 fragments would be produced. II. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 6 fragments would be produced.
Detailed explanation-3: -Restriction enzymes with shorter recognition sequences cut more frequently than those with longer recognition sequences. For example, a 4 base pair (bp) cutter will cleave, on average, every 44 (256) bases, while a 6 bp cutter cleaves every 46 (4096) bases.