OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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If a plasmid vector is digested with Eco R I at a single site then
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one sticky end will be produced
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two sticky ends will be produced
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four sticky ends will be produced
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six sticky ends will be produced
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Explanation:
Detailed explanation-1: -So, the correct option is ‘two sticky ends will be produced’.
Detailed explanation-2: -Digestion of composite NR1 DNA by EcoRI yields thirteen fragments.
Detailed explanation-3: -Answer and Explanation: a. When the vector DNA is cleaved using EcoRI, it will digest the DNA, resulting in sticky overhangs. The digested DNA can be observed and compared by using molecular size ladders on an agarose gel stained with ethidium bromide.
There is 1 question to complete.