OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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cut open plasmid DNA
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insert plasmids into bacteria
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isolate the DNA making up a human gene
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join human DNA to plasmid DNA
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Detailed explanation-1: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.
Detailed explanation-2: -DNA ligase is used in genetic engineering to generate recombinant DNA. First, two pieces of DNA, perhaps a plasmid and genomic DNA, are cut using the same restriction enzymes. Since some restriction enzymes cleave DNA unevenly, they generate single stranded regions known as sticky ends.
Detailed explanation-3: -DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.
Detailed explanation-4: -The DNA ligase catalyzes the formation of covalent phosphodiester linkages, which permanently join the nucleotides together. After ligation, the insert DNA is physically attached to the backbone and the complete plasmid can be transformed into bacterial cells for propagation.
Detailed explanation-5: -Recombination is the process through which a new gene is inserted into a bacterial DNA “The plasmid". The DNA needs to be cut with an enzyme called a restriction enzyme.