OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Cut DNA at a specific site
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Can be used to isolate a gene of interest
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Can be used to cut vector DNA
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Do all of these things
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Detailed explanation-1: -A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.
Detailed explanation-2: -The restriction enzyme is a protein produced by bacteria that cleaves the DNA at specific sites. This site is known as the restriction site. The restriction enzymes protect the live bacteria from bacteriophages. They recognize and cleave at the restriction sites of the bacteriophage and destroy its DNA.
Detailed explanation-3: -Many restriction enzymes make staggered cuts, producing ends with single-stranded DNA overhangs. However, some produce blunt ends. DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA.
Detailed explanation-4: -They are nucleases in nature. They cleave both polynucleotide strands of DNA at a specific sequence of nitrogenous bases. These sequences form recognition sites, restriction sites, or target sites. These sites have palindromic sequences of nitrogenous bases. More items