LIFE SCIENCE

Detailed explanation-1: -A restriction enzyme that cuts the backbones of both strands at non-adjacent locations leaves a staggered cut, generating two overlapping sticky ends, while an enzyme that makes a straight cut (at locations directly across from each other on both strands) generates two blunt ends.

Detailed explanation-2: -The sticky ends, a.k.a. cohesive ends, have unpaired DNA nucleotides on either 5’-or 3’-strand, which are known as overhangs. These overhangs are most often generated by a staggered cut of restriction enzymes.

Detailed explanation-3: -A ‘sticky’ end is produced when the restriction enzyme cuts at one end of the sequence, between two bases on the same strand, then cuts on the opposite end of the complementary strand. This will produce two ends of DNA that will have some nucleotides without any complementary bases.

Detailed explanation-4: -Each enzyme recognizes one or a few target sequences and cuts DNA at or near those sequences. Many restriction enzymes make staggered cuts, producing ends with single-stranded DNA overhangs.

Detailed explanation-5: -Restriction enzymes are used to cut DNA at specific sequences. The cut can leave sticky ends, meaning there is an overhang of bases. These bases are useful because it allows scientists to specifically match up pieces of DNA. EcoRI is a restriction enzyme that makes sticky ends.