LIFE SCIENCE

Detailed explanation-1: -The first libraries were cloned in simple plasmids and phage vectors. Explanation: Genomic libraries are useful to examine how we can study and isolate a given gene; the basic principles apply to all genomes.

Detailed explanation-2: -A genomic library is a collection of overlapping segments of genomic DNA, cloned into a backbone vector, which statistically includes all regions of the genome of an organism. The resulting cloned DNA is then transformed into a suitable host cell line.

Detailed explanation-3: -The Genomic Library or gene bank is constructed by a shotgun experiment where the entire genome of the cell is cloned in the form of random and unidentifiable clones. It uses the chain termination method (Sanger’s sequencing) to sequence the DNA molecules.

Detailed explanation-4: -In order to construct a genomic library, the organism’s DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase.

Detailed explanation-5: -Isolate a sample of DNA from the organisms cells. Cut the DNA into “gene-size” pieces with a restriction enzyme or enzymes. Cut a cloning plasmid with the same restriction enzymes. Add the cut plasmid and genomic DNA to the same test tube and add DNA ligase. More items