OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Cutting and ligation of DNA at specific locations
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Amplification of gene of interest using PCR
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Isolation of the genetic material or DNA
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Insertion of recombinant DNA into the host cell or organism
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Detailed explanation-1: -The first step in rDNA technology is to isolate the desired DNA in its pure form i.e. free from other macromolecules. However, in a normal cell, the DNA not only exists within the cell membrane, but is also present along with other macromolecules such as RNA, polysaccharides, proteins, and lipids.
Detailed explanation-2: -The first step in the development of recombinant DNA technology was the characterization of restriction endonucleases-enzymes that cleave DNA at specific sequences. These enzymes were identified in bacteria, where they apparently provide a defense against the entry of foreign DNA (e.g., from a virus) into the cell.
Detailed explanation-3: -Recombinant DNA technology involves the selection of the desired gene for administration into the host followed by a selection of the perfect vector with which the gene has to be integrated and hence the recombinant DNA is formed. This recombinant DNA, then has to be introduced into the host.
Detailed explanation-4: -Isolated DNA is first cut into readily separable fragments with restriction nucleases. The double-stranded fragments are then separated on the basis of size by gel electrophoresis, and those complementary to a DNA probe are identified by blotting and hybridization, as just described for RNA (see Figure 8-27).
Detailed explanation-5: -Table of Contents. Also Refer-Genes. Also Read: Bioinformatics. Step-1. Isolation of Genetic Material. Step-2.Cutting the gene at the recognition sites. Step-3. Amplifying the gene copies through Polymerase chain reaction (PCR). Step-4. Ligation of DNA Molecules. Step-5. More items •14-Apr-2020