LIFE SCIENCE

Detailed explanation-1: -Molecular cloning is the collection of experimental procedures required to isolate and expand a specific fragment of DNA into a host organism in order to create a large number of identical copies.

Detailed explanation-2: -DNA cloning is a molecular biology technique that makes many identical copies of a piece of DNA, such as a gene.

Detailed explanation-3: -Step 4. The vector DNA is isolated (or separated) from the host cells’ DNA and purified. DNA that has been ‘cut’ and ‘pasted’ from an organism into a vector is called recombinant DNA. Because of this, DNA cloning is also called recombinant DNA technology.

Detailed explanation-4: -recombinant DNA technology / DNA cloning; gene cloning; cloning. A technology that uses enzymes to cut and paste together DNA sequences of interest. The recombined DNA sequences can be placed into vectors that carry the DNA into a host cell.

Detailed explanation-5: -To be useful, the recombinant molecule must be replicated many times to provide material for analysis, sequencing, etc. Producing many identical copies of the same recombinant molecule is called cloning. Cloning can be done in vitro, by a process called the polymerase chain reaction (PCR).