LIFE SCIENCE

Detailed explanation-1: -Sticky end is a fragment of DNA produced by a staggered cut using restriction enzymes, in which the terminal portion has a stretch of unpaired bases. Examples include EcoR I, Hind III, BamH I etc.

Detailed explanation-2: -The sticky ends, a.k.a. cohesive ends, have unpaired DNA nucleotides on either 5’-or 3’-strand, which are known as overhangs.

Detailed explanation-3: -A ‘sticky’ end is produced when the restriction enzyme cuts at one end of the sequence, between two bases on the same strand, then cuts on the opposite end of the complementary strand. This will produce two ends of DNA that will have some nucleotides without any complementary bases.

Detailed explanation-4: -The sticky ends form base pairs. Any two complementary cohesive ends can anneal, even those from two different organisms. This bondage is temporary however, and DNA ligase will eventually form a covalent bond between the sugar-phosphate residue of adjacent nucleotides to join the two molecules together.

Detailed explanation-5: -The answer is A: the single-stranded ends of a DNA segment created by some restriction enzymes. Sticky ends are short, overhangs of DNA created by specific restriction enzymes (like EcoRI) that create this type of end.