LIFE SCIENCE

Detailed explanation-1: -Recombinant human insulin was first produced in E. coli by Genentech in 1978, using a approach that required the expression of chemically synthesized cDNA encoding for the insulin A and B chains separately in E. coli [59].

Detailed explanation-2: -Recombinant insulin is produced using Escherichia coli bacteria. It is produced by cloning genes of human A and B peptides of insulin separately in different E. coli bacteria. At the end, disulfide bonds are created in between both the newly created peptides to form functional insulin.

Detailed explanation-3: -coli by recombinant DNA technology. Synthetic genes for human insulin A and B chains are cloned separately in plasmid pBR322. The cloned synthetic genes are then fused to an Escherichia coli -galactosidase gene to provide efficient transcription and translation and a stable precursor protein.

Detailed explanation-4: -The first genetically engineered, synthetic “human” insulin was produced in 1978 using E. coli bacteria to produce the insulin. Eli Lilly went on in 1982 to sell the first commercially available biosynthetic human insulin under the brand name Humulin.