LIFE SCIENCE

Detailed explanation-1: -DNA cloning allows a copy of any specific part of a DNA or RNA sequence to be selected from the millions of other sequences in a cell and produced in unlimited amounts in pure form.

Detailed explanation-2: -DNA cloning. In DNA cloning, researchers “clone” – make many copies of – a DNA fragment of interest, such as a gene. In many cases, DNA cloning involves inserting a target gene into a circular DNA molecule called a plasmid. The plasmid can be replicated in bacteria, making many copies of the gene of interest.

Detailed explanation-3: -PCR (polymerase chain reaction) is a method used in molecular biology to make millions of physical copies of a specific DNA sequence, for example, a gene. It has several key ingredients: a DNA template to copy, short DNA sequences called “primers”, and a master mix containing the rest of necessary molecules.

Detailed explanation-4: -PCR (Polymerase Chain Reaction) is a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand.

Detailed explanation-5: -Polymerase chain reaction (PCR) is a technique used to rapidly increase the number of copies of specific regions of DNA for further analyses (Figure 10.4).